ブックタイトル神戸女子大学家政学部紀要 第50巻

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神戸女子大学家政学部紀要 第50巻

-12 -Effect of intake of milk on blood leucine level and Body fat mass in the Japanese elderlysynthesis9,10). Moreover, muscle damage inducedby exercise is reduced by the supplementation ofBCAA11,12).In this study, we investigated the effects of intake ofmilk containing a lot of BCAA and compared betweenbefore and after the intake of milk in body compositionand blood analysis in the Japanese elderly. Our basicresearch findings will support the promotion of milkintake and exercise for prevention of sarcopenia, whichis a critical challenge for us in rapidly aging Japanesesociety.MethodsWe carried out this study in accordance with theDeclaration of Helsinki. This study was approved andcarried out according to the guidelines of the ethicscommittee of human research of Wayo Women’sUniversity. The subjects gave written consent toparticipate in the study. This study was carried out in2014.Subjects and body compositionSubjects in the present study were 22 healthyelderly individuals (15 females and 7 males) aged from67 to 87 years (76.9±5.0: Mean±S.D.), who lived inKanto area of Japan. Weight, lean mass, muscle mass,muscle mass percentage, fat mass, body fat percentage,and estimated bone mass were measured by multifrequencysegmental body composition analyzer(TANITA MC-190). Height was measured and bodymass index (BMI, kg/m2) was calculated.Analysis of Food and nutrient intakeBr ief - type sel f -admini s tered diet hi s toryquestionnaire (BDHQ) was conducted for analyzingfood, energy and nutrient intakes13-15). The subjectswere requested to answer the questionnaires whichcontain the kinds of food, amount and the frequencythe subjects had for the previous one month of theinvestigation. 4 page sheets filled by each subject wassent to the EBN JAPAN (DHQ Support Center, Tokyo,Japan) which analyzed energy intake, nutrient intake,kind of food and amount of food.Intake of milk and blood analysisSubjects took 180ml/day regular milk for twomonths. The timing of taking milk was not defined.Before and after the intake of milk, blood samplingwas carried out approximately 2 hours after breakfast.Blood was analyzed by SRL Co., Ltd. (Tokyo, Japan).Statistical analysisBody composition and blood analysis values werecompared between before and after the intake of milk.Two-tailed paired student’s t-test was performed andp<0.05 was considered significantly different.ResultsBody compositionTable1 shows body composition before and afterthe intake of milk. The values of weight (56.8±9.8kg),BMI (24.1±2.2kg/m2), fat mass (18.5±4.3kg), bodyfat percentage (32.6±5.4%) before the intake ofmilk significantly decreased after the intake of milk(55.4±9.6kg, 23.5±2.2 kg/m2, 17.1±4.5kg, 30.8±6.0%,respectively) (p<0.05). Lean body mass (39.3±7.6kgvs. 39.3±7.6kg), muscle mass (37.2±7.3kg vs.37.2±7.2kg), and estimated bone mass (2.1±0.4kgvs. 2.1±0.4kg), were the same before and after theintake of milk. The values of muscle mass percentage(67.2±5.8%) after the intake of milk were significantlyhigher than the values before the intake of milk(65.5±5.2%) (p<0.05).Analysis of Food and nutrient intakeNutrient intake was shown in Table2. Energyintake before the intake of milk (1920.5±624.9kcal/day) and carbohydrate (266.6±99.6g/day) decreasedafter the intake of milk (1860.6±663.3kcal/day,240.2±96.9g/day, respectively). The intake of protein(77.1±25.3g/day), mineral (20.6±5.4g/day), cholesterol(407.8±137.9mg/day) increased after the intake ofmilk (85.3±39.6g/day, 23.3±9.6g/day, 483.7±305.5mg/day, respectively), though there was no significantdifference. Food intake was compared before and afterthe intake of milk (Table3). The values of green andyellow vegetables, other vegetables, beans, sea foods,eggs, dairy, sweets increased after the intake of milk,but there was no significant difference. The values oftubers, oils and fats, fruits decreased after the intakeof milk. The intake of cereals decreased significantlyafter the intake of milk (433.7±203.3g/day vs.346.1±133.7g/day) (p<0.05).Analysis of bloodAnalysis of blood was shown in Table4. The valuesof serum albumin, total cholesterol, LDL-cholesterol,HDL-cholesterol, triglyceride and calcium were almostthe same before and after the intake of milk. Table5