ブックタイトル神戸女子大学家政学部紀要 第50巻

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神戸女子大学家政学部紀要 第50巻

-14 -Effect of intake of milk on blood leucine level and Body fat mass in the Japanese elderlyshowed the values of essential amino acids in serum.The values of threonine, methionine, histidine andtryptophan, were almost the same before and after theintake of milk. The values of valine, phenylalanine,isoleucine and lysine increased after the intake ofmilk, though there was no significant difference. TotalBCAA, valine, leucine, isoleucine increased afterthe intake of milk, and especially leucine increasedsignificantly (115.9±25.4nmol/ml vs. 128.6±21.3nmol/ml) (p<0.05).DiscussionIn the present study, we tried to show basic researchresults for the formation of public health policy for theprevention of sarcopenia. Intake of 180ml regular milka day for two months increased leucine level in theelderly. Many studies in humans and animals reportedthat the intake of leucine in the meal stimulated proteinsynthesis in skeletal muscle via rapamycine-sensitivepathway, and the peak of protein synthesis was 30to 60 minutes after leucine administration16-21). Ourresults showed that the intake of milk for two monthsincreased significantly leucine level in serum, but theaverage of muscle mass was exactly the same after theintake of milk. Shad et al. have shown that more intakeof leucine would be required to respond to exercise inthe elderly in comparison with the protein synthesisin the young9,22). Muscle protein synthesis should bestimulated and muscle mass should increase, if theolder subjects intake more milk along with exercises.We will confirm this hypothesis in the next study.Our results indicated that fat mass and weightdecreased significantly after the intake of milk.Jiao et al. study using animals showed that leucinesupplementation decreased lipogenic enzymessuch as fatty acids synthase and acetyl-coenzymeA carboxylase leading to the improvement of lipidmetabolism23). The results in this study could beexplained by this mechanism. However, the significantdecrease of cereal after intake of milk might be thereason for the decrease of fat mass. It was reported thatleucine supplementation enhanced leptin sensitivity bypromoting leptin signaling24). Fat mass decreased bythe intake of milk, because leucine supplementationdecreased fat mass directly, or leucine decreased intakeof cereals by enhanced leptin sensitivity leading toTable5. Blood analysis in essential amino acidsBefore intake of milk After intake of milkThreonine (nmol/ml) 124.7 ± 30.8 130.7 ± 26.8Valine (nmol/ml) 227.9 ± 39.2 236.0 ± 36.4Methionine (nmol/ml) 27.0 ± 6.1 28.5 ± 6.2Leucine (nmol/ml) 115.9 ± 25.4 128.6 ± 21.3*Phenylalanine (nmol/ml) 61.9 ± 11.0 66.7 ± 10.6Histidine (nmol/ml) 78.0 ± 7.8 77.7 ± 8.9Tryptophan (nmol/ml) 54.6 ± 11.4 55.3 ± 10.1Isoleucine (nmol/ml) 67.9 ± 16.9 72.0 ± 15.9Lysine (nmol/ml) 199.9 ± 40.2 207.4 ± 32.5Total BCAA (nmol/ml) 411.6 ± 78.2 436.6 ± 71.0Mean±S.D.* ; Significant difference between before and after the intake of milk (p<0.05)BCAA; branched chain amino acidTable4. Blood analysisBefore intake of milk After intake of milkAlbumin (g/dl) 4.3 ± 0.3 4.3 ± 0.3Total cholesterol (mg/dl) 210.0 ± 33.4 213.7 ± 35.5LDL-cholesterol (mg/dl) 122.4 ± 33.2 125.9 ± 31.3HDL-cholesterol (mg/dl) 59.7 ± 13.9 60.3 ± 14.5Triglyceride (mg/dl) 152.1 ± 51.5 155.9 ± 63.5Calcium (mg/dl) 9.5 ± 0.3 9.5 ± 0.4Mean±S.D.